Jiujie Machine
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The Fluo-100 is a dual-channel fluorometer, providing highly sensitive fluorescent detection when quantifying nucleic acids and proteins. Simple and cost-effective to operate.
Our nano series Micro-Spectrophotometers measure the concentration and purity of the sample by measuring the absorbance of the whole ingredient (such as DNA, RNA, oligos, etc.) at 260nm, which may occasionally lead to inaccurate readings.
In contrast, the fluorometer measures the fluorescence intensity of the fluorescent reagent that specifically combines with the target molecule to read the exact concentration of the sample. Generally, the reading is lower than Nano series products but significantly more accurate.
The sensitivity of the Nano series products is above 5ng/μl, whereas the fluorometer achieves 0.5pg/μl in measuring DNA concentration. Reading lower and highly accurate concentrations of the sample is crucial for most applications. Using the Fluo-100 fluorometer with the quantitation kit allows rapid, sensitive, and accurate sample concentration measurement.
| Light source | LED |
| Dynamic range | Five orders of magnitude |
| Linear Dynamic Range | R >0.995 |
| Detector | Photodiode |
| Repeatability | <1.5% |
| Stability | <1.5% |
| Sensitivity | dsDNA: 0.5ng/ml |
| Measurement speed | 3s (Once) |
| Dimension (W×D×H) | 194×155×72.5 mm |
| Weight | 0.4 Kg |
| Ordering Information Table | |||
|---|---|---|---|
| Code | Description | Code | Description |
| AS-18010-00 | Fluo-100A Fluorometer, DC12V 1A | RS-FL0101-S RS-FL0101-M RS-FL0101-L |
High-sensitivity dsDNA Quantitation kits (100 / 200 / 1000 assays) |
| AS-18020-00 | Fluo-100B Fluorometer, DC12V 1A | ||
| AS-18030-00 | Fluo-100C Fluorometer, DC12V 1A | RS-FL0102-S RS-FL0102-M RS-FL0102-L |
Broad-range dsDNA Quantitation kits (100 / 200 / 1000 assays) |
| AS-18011-01 | Adapter for 0.5ml qPCR tube (Standard) | ||
| AS-18011-02 | Adapter for 0.2ml qPCR tube (Options) | RS-FL0103-S RS-FL0103-M RS-FL0103-L |
High-sensitivity ssDNA Quantitation kits (100 / 200 / 1000 assays) |
The Fluo-100 offers a straightforward, easy-to-use protocol. The workflow integrates seamlessly with quantification assays to provide accurate fluorescent readouts.
| Model | Light | Excitation Filters | Emission Filters |
|---|---|---|---|
| Fluo-100A | UV | 365±20nm | 420-480nm (60nm) |
| Blue | 460±20nm | 525-570nm (45nm) | |
| Fluo-100B | Blue | 460±20nm | 525-570nm (45nm) |
| Red | 625±10nm | 670-725nm (55nm) | |
| Fluo-100C | Blue | 460±20nm | 525-570nm (45nm) |
| Green | 525±20nm | 575-640nm (65nm) |
| Channel | Excitation Filters | Normal Kits | Applications |
|---|---|---|---|
| UV channel | 365±20nm | Hoechst 33258, 4-MU, EnZCheK Caspase | Nucleic acid quantification, Plant GUS reporter gene detection, Apoptosis detection |
| Blue channel | 460±20nm | PicoGreen®, oligreen, RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™ | dsDNA, ssDNA, RNA quantification, GFP gene detection, Fluorescein detection, Protein detection |
| Green channel | 525±20nm | Rhodamine, Cy3, RFP Vybrant Cytotoxicity | Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, Cell activity poison detection |
| Red channel | 625±10nm | Cy5, Quant-iT RNA | Cy-5 fluorescence labeling detection, RNA quantification |
